1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Related Products (936):

Cat. No. Product Name Effect Purity
  • HY-17393
    Carboplatin Inhibitor
    Carboplatin (NSC 241240) is a DNA synthesis inhibitor which binds to DNA, inhibits replication and transcription and induces cell death. Carboplatin (NSC 241240) is a derivative of CDDP and a potent anti-cancer agent.
  • HY-17565
    Bleomycin sulfate Inhibitor
    Bleomycin sulfate is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin sulfate is an antitumor antibiotic[1].
  • HY-17565A
    Bleomycin hydrochloride Inhibitor
    Bleomycin hydrochloride is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin hydrochloride is an antitumor antibiotic[1].
  • HY-17026
    Gemcitabine Inhibitor
    Gemcitabine (LY 188011) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine inhibits DNA synthesis and repair, resulting in autophagyand apoptosis[1][2].
  • HY-17371
    Oxaliplatin Inhibitor
    Oxaliplatin is a DNA synthesis inhibitor. Oxaliplatin causes DNA crosslinking damage, prevents DNA replication and transcription and induces apoptosis. Oxaliplatin can be used for cancer research[1][2][3].
  • HY-160884
    DMT-2'-OMe-D-Ribitol phosphoramidite
    DMT-2'-OMe-D-Ribitol phosphoramidite (compound 9) is an abasic phosphoramidite monomer that can be used to introduce the abasic group Y34 into oligonucleotides[1].
  • HY-129246
    Cytembena Inhibitor
    Cytembena is inhibitor for replicative DNA synthesis, purine synthesis and tetrahydrofolate formylase activity. Cytembena ameliorates ovarian or breast cancer, relieves the pain from skeletal metastases[1][2].
  • HY-P10328
    Vasoactive intestinal contractor Inhibitor
    Vasoactive intestinal contractor is a novel member of the endothelin family. Vasoactive intestinal contractor stimulates a rapid increase in the intracellular Ca2+ concentration in fura-2-overexpressed Swiss 3T3 cells[1.
  • HY-162146
    NS3-IN-1 Inhibitor
    NS3-IN-1 (compound 302) is a potent helicase (NS3) inhibitor. NS3-IN-1 shows an potent inhibitor for yellow fever helicase[1].
  • HY-12695B
    Guanosine 5'-triphosphate trisodium salt hydrate
    5'-GTP trisodium salt hydrate is an activator of the signal transducing G proteins and also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA.
  • HY-133887
    Methotrexate α-tert-butyl ester Inhibitor
    Methotrexate α-tert-butyl ester, capped by OtBu, significantly reduces tumor growth in HT1080 tumor bearing mice. Methotrexate is an antimetabolite and antifolate agent and is also an immunosuppressant and antineoplastic agent[1][2][3][4].
  • HY-W048479
    7-Iodo-2',3'-dideoxy-7-deaza-guanosine 99.17%
    7-Iodo-2',3'-dideoxy-7-deaza-guanosine is a dideoxynucleoside that can be used in DNA synthesis and sequencing reactions[1].
  • HY-145538
    2'-Deoxyguanosine 5'-monophosphate disodium hydrate
    2'-Deoxyguanosine 5'-monophosphate (5'-Deoxyguanylic acid; dGMP) disodium hydrate is an oxidizable target of the photosensitizer pterin (PT) and can be used to evaluate the photosensitizing properties of biopterins (such as Bip, Fop and Cap) . Pterin causes a photosensitive reaction of dGMP under UV-A radiation, causing damage to DNA molecules. There are two main mechanisms for the photosensitive oxidation of purine nucleotides by pterin in vitro: one is the hydrogen abstraction reaction of electron transfer from dGMP to the triplet excited state of pterin (type I mechanism), and the other is the interaction between dGMP and pterin. The reaction produces singlet molecular oxygen (1O2) (Type II mechanism)[1][2].
  • HY-16637A
    Folic acid sodium
    Folic acid (Vitamin B9) sodium is a orally active essential nutrient from the B complex group of vitamins. Folic acid sodium shows antidepressant-like effect. Folic acid sodium reduces the risk of neonatal neural tube defects. Folic acid sodium can be used to the research of megaloblastic and macrocytic anemias due to folic deficiency[1][2][3][4].
  • HY-W141393
    2'-OMe-dmf-G-CE-Phosphoramidite is a phosphorite monomer that can be used in the synthesis of oligonucleotides.
  • HY-16035
    Alatrofloxacin Inhibitor
    Alatrofloxacin, the parenteral proagent of Trovafloxacin, is a fluoronaphthyridone which contains an L-alanyl-L-alanyl salt. Alatrofloxacin functions similar to other fluoroquinolone antibiotics in that it not only has antibiotic activity to kill invading organisms by interfering with DNA synthesis, it possesses immunosuppressive activity[1].
  • HY-W042357
    Ac-rC Phosphoramidite 98.87%
    Ac-rC Phosphoramidite is used for the oligoribonucleotide phosphorodithioate modification (PS2-RNA)[1].
  • HY-128917
    DNA31 Inhibitor 98.20%
    DNA31 is a potent RNA polymerase inhibitor[1].
  • HY-107845
    SCR7 pyrazine Inhibitor 99.96%
    SCR7 pyrazine is a DNA ligase IV inhibitor that blocks nonhomologous end-joining (NHEJ) in a ligase IV-dependent manner. SCR7 pyrazine is also a CRISPR/Cas9 enhancer which increases the efficiency of Cas9-mediated homology-directed repair (HDR). SCR7 pyrazine induces cell apoptosis and has anticancer activity[1][2].
  • HY-154031
    3'-F-3'-dA(Bz)-2'-Phosphoramidite is a phosphorite monomer that can be used in the synthesis of oligonucleotides.