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Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Fluorescent Dye Related Products (1206):

Cat. No. Product Name Effect Purity
  • HY-15924
    Thiazolyl Blue
    Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis[1].
  • HY-111382
    Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios[1].
  • HY-D0718
    Nile Red 98.15%
    Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1].
  • HY-15534
    JC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=585nm, Em=590nm); When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=514nm, em=529nm)[1].
  • HY-D0150
    Thiazole Orange 99.78%
    Thiazole orange is an unsymmetrical cyanine dye which can be conjugated to oligonucleotides (ONs) to create fluorogenic hybridisation probes. Thiazole orange can be used for reticulocyte analysis[1][2].
  • HY-D1421
    PKH 67
    PKH67 is a fluorescent cell binding dye with green fluorescence. PKH67 can stain the cell membrane and the Ex/Em is 490/502 nm. PKH67 is often used in combination with the non-specific red fluorescent dye PKH26 (Ex/Em=551/567 nm) to label cells, detect cell proliferation in vitro, and trace cells in vitro and in vivo[1][2].
  • HY-153081
    eGFP circRNA
    The eGFP circRNA will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. The eGFP circRNA is therefore ideal for monitoring and optimizing transfection efficiency and is recommended as a positive control for circular RNA transfections.
  • HY-W040144
    Bromocresol green
    Bromocresol green is a pH-sensitive triphenylmethane dye commonly used for the determination of protein and albumin in serum. Bromocresol green is a bio-based dye with a yellow-green to blue-green color. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in acidic solution (e.g. pH=4.15), and turns blue in basic solution (λmax=615 nm, deprotonated form). Bromocresol green is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells[1][2][3][4].
  • HY-D1269
    Rhodamine-N3 chloride
    Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups[1][2].
  • HY-151701
    DiSulfo-Cy5 alkyne
    DiSulfo-Cy5 alkyne is a fluorescent dye can be used as a click chemistry reagent[1].
  • HY-135636
    Ponatinib Acid
    Ponatinib Acid, an analogue of Ponatinib, is usually used as a labeled chemical or fluorescent probe.
  • HY-D1029A
    Biotin-11-dUTP trisodium
    Biotin-11-dUTP trisodium is a fluorescent substitute for dTTP.
  • HY-W151206
    Hydroxy naphthol blue disodium
    Hydroxy naphthol blue disodium, an azo dye, is a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions ( λmax = 650 nm). In the pH range between 12 and 13, the solution of the indicator is reddish pink in the presence of calcium ion and to deep blue in the presence of disodium EDTA[1].
  • HY-D1030
    Fluorescein Biotin
    Fluorescein Biotin is used as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance; the the fluorescence or absorbance of Fluorescein Biotin is quenched, upon binding to avidin or streptavidin.
  • HY-D1646
    BDP TR methyltetrazine
    BDP TR methyltetrazine is a BDP dye linker containing a methyltetrazine group.
  • HY-D0099
    Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives[1][2].
  • HY-D1719
    Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging[1][2].
  • HY-100041
    Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols[1][2].
  • HY-137296
    Lumogallion 99.73%
    Lumogallion is a highly sensitive fluorescent reagent for the detection of aluminum, gallium and other metals. Lumogallion has an excitation wavelength of 490 nm and an emission spectrum in the range of 520 nm to 650 nm, with a peak near 580 nm[1][2].
  • HY-127008
    N-Butylfluorescein is an alkyl-substituted fluorescein, can be used for synthesis of fluorogenic substrates for assaying phosphatidylinositol-specific phospholipase C[1].